[PDF/Kindle] CRISPR-Cas: A Laboratory Manual by Jennifer Doudna

CRISPR-Cas: A Laboratory Manual. Jennifer Doudna

CRISPR-Cas-A-Laboratory.pdf
ISBN: 9781621821311 | 450 pages | 12 Mb

• CRISPR-Cas: A Laboratory Manual
• Jennifer Doudna
• Page: 450
• Format: pdf, ePub, fb2, mobi
• ISBN: 9781621821311
• Publisher: Cold Spring Harbor Laboratory Press

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Pdf download ebook free CRISPR-Cas: A Laboratory Manual by Jennifer Doudna FB2 RTF English version 9781621821311

CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

Supplemental Material - RNA
Expression of CRISPR/Cas single guide RNAs using small tRNA promoters Molecular Cloning: A Laboratory Manual (Fourth Edition)  Addgene: Zhang Lab CRISPR Page
CRISPR Cas9 gRNA plasmids from the Zhang lab. Zhang lab CRISPR icon Zhang Lab CRISPR Plasmids Available from Addgene  CRISPR Systems - Santa Cruz Biotechnology
CRISPR Cas9 dCas9 gene editing plasmids for gene knockout and gene Lab Supplies The CRISPR/Cas system is an adaptive immune defense mechanism used by Archea and bacteria for the degradation of foreign genetic material. Crispr-Cas - BookManager
Other editions for: Crispr-Cas Crispr-Cas: A Laboratory Manual | Paperback The Use of Crispr/Cas9, Zfns, Talens in Generating Site Specific Genome  Cold Spring Harbor Lab Press Books
Edited By Bruce Stillman, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory; Terri Grodzicker, CRISPR-Cas: A Laboratory Manual

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